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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which leads to chromosome instability, and so may contribute to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial part in marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be described. Two additional splice variants were claimed but specific nucleotide sequence facts is just not but obtainable.
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Blocking peptides are peptides that bind specially on the target antibody and block antibody binding. These peptide normally consists of the epitope regarded via the antibody.
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This manual outlines even further achievable optimization of cellular and immunoassay parameters to ensure the absolute best results are...
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This manual outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope around the goal protein. By evaluating the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with read more and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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